Journal: Cancer Management and Research

Article Title: Tumor-Associated CD163 + M2 Macrophage Infiltration is Highly Associated with PD-L1 Expression in Cervical Cancer

doi: 10.2147/CMAR.S257692

Figure Lengend Snippet: Immunohistochemistry staining for PD-L1, CD68, and CD163 expression in cervical squamous cell carcinoma tissues. The left panel shows negative staining for PD-L1 and low macrophage density. The right panel shows positive staining for PD-L1 and high macrophage density.

Article Snippet: After placing in a microwave oven for pretreatment (PD-L1: EDTA antigen repair solution for 30 min, pH 9; CD68 and CD163: sodium citrate buffer for 15 min, pH 6), endogenous peroxidase was inhibited by 3% H 2 O 2 for 30 min, then the sections were incubated with 10% normal goat serum for 40 min. Primary antibodies composed of rabbit anti-PD-L1 antibody 28–8 (diluted 1:100, ab205921, Abcam, Cambridge, UK), rabbi anti-CD68 antibody (diluted 1:50, BA3638, Boster, California, UK), or rabbit anti-CD163 antibody (diluted 1:200, bs-2527R, bioss, Beijing, China), were applied overnight in a moist chamber at 4°C.

Techniques: Immunohistochemistry, Staining, Expressing, Negative Staining

Journal: Cancer Management and Research

Article Title: Tumor-Associated CD163 + M2 Macrophage Infiltration is Highly Associated with PD-L1 Expression in Cervical Cancer

doi: 10.2147/CMAR.S257692

Figure Lengend Snippet: Density of CD68- and CD163-Positive Cells in the Tumor Field and Clinical Features in 120 Samples of Cervical Squamous Cell Carcinoma

Article Snippet: After placing in a microwave oven for pretreatment (PD-L1: EDTA antigen repair solution for 30 min, pH 9; CD68 and CD163: sodium citrate buffer for 15 min, pH 6), endogenous peroxidase was inhibited by 3% H 2 O 2 for 30 min, then the sections were incubated with 10% normal goat serum for 40 min. Primary antibodies composed of rabbit anti-PD-L1 antibody 28–8 (diluted 1:100, ab205921, Abcam, Cambridge, UK), rabbi anti-CD68 antibody (diluted 1:50, BA3638, Boster, California, UK), or rabbit anti-CD163 antibody (diluted 1:200, bs-2527R, bioss, Beijing, China), were applied overnight in a moist chamber at 4°C.

Techniques: Biomarker Discovery

Journal: Cancer Management and Research

Article Title: Tumor-Associated CD163 + M2 Macrophage Infiltration is Highly Associated with PD-L1 Expression in Cervical Cancer

doi: 10.2147/CMAR.S257692

Figure Lengend Snippet: Correlation of PD-L1 expression and density of CD68-and CD163-positive cells in cervical cancer. Notes: ( A ) PD-L1 expression in tumor cells and density ofCD68. ( B ) PD-L1 expression in tumor cells and density of CD163. ( C ) PD-L1 expression in the stroma and density of CD68. ( D ) PD-L1 expression in the stroma and density of CD163.

Article Snippet: After placing in a microwave oven for pretreatment (PD-L1: EDTA antigen repair solution for 30 min, pH 9; CD68 and CD163: sodium citrate buffer for 15 min, pH 6), endogenous peroxidase was inhibited by 3% H 2 O 2 for 30 min, then the sections were incubated with 10% normal goat serum for 40 min. Primary antibodies composed of rabbit anti-PD-L1 antibody 28–8 (diluted 1:100, ab205921, Abcam, Cambridge, UK), rabbi anti-CD68 antibody (diluted 1:50, BA3638, Boster, California, UK), or rabbit anti-CD163 antibody (diluted 1:200, bs-2527R, bioss, Beijing, China), were applied overnight in a moist chamber at 4°C.

Techniques: Expressing

Journal: Cancer Management and Research

Article Title: Tumor-Associated CD163 + M2 Macrophage Infiltration is Highly Associated with PD-L1 Expression in Cervical Cancer

doi: 10.2147/CMAR.S257692

Figure Lengend Snippet: Relationship between the density of CD68- and CD163-positive cells and the expression of PD-L1 in cervical cancer. ( A ) Density of CD68 in PD-L1 positive and negative expression groups in TC. ( B ) Density of CD163 in PD-L1 positive and negative expression groups in TC. ( C ) Density of CD68 in PD-L1 positive and negative expression groups in stroma. ( D ) Density of CD163 in PD-L1 positive and negative expression groups in stroma.

Article Snippet: After placing in a microwave oven for pretreatment (PD-L1: EDTA antigen repair solution for 30 min, pH 9; CD68 and CD163: sodium citrate buffer for 15 min, pH 6), endogenous peroxidase was inhibited by 3% H 2 O 2 for 30 min, then the sections were incubated with 10% normal goat serum for 40 min. Primary antibodies composed of rabbit anti-PD-L1 antibody 28–8 (diluted 1:100, ab205921, Abcam, Cambridge, UK), rabbi anti-CD68 antibody (diluted 1:50, BA3638, Boster, California, UK), or rabbit anti-CD163 antibody (diluted 1:200, bs-2527R, bioss, Beijing, China), were applied overnight in a moist chamber at 4°C.

Techniques: Expressing

Journal: Cancer Management and Research

Article Title: Tumor-Associated CD163 + M2 Macrophage Infiltration is Highly Associated with PD-L1 Expression in Cervical Cancer

doi: 10.2147/CMAR.S257692

Figure Lengend Snippet: Univariate and Multivariate Analysis of Factors Associated with PD-L1 Expression in Tumor Cells

Article Snippet: After placing in a microwave oven for pretreatment (PD-L1: EDTA antigen repair solution for 30 min, pH 9; CD68 and CD163: sodium citrate buffer for 15 min, pH 6), endogenous peroxidase was inhibited by 3% H 2 O 2 for 30 min, then the sections were incubated with 10% normal goat serum for 40 min. Primary antibodies composed of rabbit anti-PD-L1 antibody 28–8 (diluted 1:100, ab205921, Abcam, Cambridge, UK), rabbi anti-CD68 antibody (diluted 1:50, BA3638, Boster, California, UK), or rabbit anti-CD163 antibody (diluted 1:200, bs-2527R, bioss, Beijing, China), were applied overnight in a moist chamber at 4°C.

Techniques: Expressing, Biomarker Discovery

Journal: Cancer Management and Research

Article Title: Tumor-Associated CD163 + M2 Macrophage Infiltration is Highly Associated with PD-L1 Expression in Cervical Cancer

doi: 10.2147/CMAR.S257692

Figure Lengend Snippet: Recurrence-free and overall survival curves in CC patients. ( A ) Kaplan–Meier OS curves according to PD-L1 expression in tumor cells ( B ) Kaplan–Meier OS curves according to PD-L1 expression in stroma ( C ) Kaplan–Meier OS curves according to the density of CD68-positive cells ( D ) Kaplan–Meier OS curves according to the density of CD163-positive cells ( E ) Kaplan–Meier RFS curves according to PD-L1 expression in tumor cells ( F ) Kaplan–Meier RFS curves according to PD-L1 expression in stroma ( G ) Kaplan–Meier RFS curves according to the density of CD68-positive cells ( H ) Kaplan–Meier RFS curves according to the density of CD163-positive cells. (The density of CD68/CD163 above median = high CD68/CD163; The density of CD68/CD163 below median = low CD68/CD163; p -values obtained from Log-rank tests.).

Article Snippet: After placing in a microwave oven for pretreatment (PD-L1: EDTA antigen repair solution for 30 min, pH 9; CD68 and CD163: sodium citrate buffer for 15 min, pH 6), endogenous peroxidase was inhibited by 3% H 2 O 2 for 30 min, then the sections were incubated with 10% normal goat serum for 40 min. Primary antibodies composed of rabbit anti-PD-L1 antibody 28–8 (diluted 1:100, ab205921, Abcam, Cambridge, UK), rabbi anti-CD68 antibody (diluted 1:50, BA3638, Boster, California, UK), or rabbit anti-CD163 antibody (diluted 1:200, bs-2527R, bioss, Beijing, China), were applied overnight in a moist chamber at 4°C.

Techniques: Expressing

Journal: Cancer Management and Research

Article Title: Tumor-Associated CD163 + M2 Macrophage Infiltration is Highly Associated with PD-L1 Expression in Cervical Cancer

doi: 10.2147/CMAR.S257692

Figure Lengend Snippet: Univariate Analysis of Overall and Recurrence-Free Survival

Article Snippet: After placing in a microwave oven for pretreatment (PD-L1: EDTA antigen repair solution for 30 min, pH 9; CD68 and CD163: sodium citrate buffer for 15 min, pH 6), endogenous peroxidase was inhibited by 3% H 2 O 2 for 30 min, then the sections were incubated with 10% normal goat serum for 40 min. Primary antibodies composed of rabbit anti-PD-L1 antibody 28–8 (diluted 1:100, ab205921, Abcam, Cambridge, UK), rabbi anti-CD68 antibody (diluted 1:50, BA3638, Boster, California, UK), or rabbit anti-CD163 antibody (diluted 1:200, bs-2527R, bioss, Beijing, China), were applied overnight in a moist chamber at 4°C.

Techniques: Expressing

Journal: Journal of biomedical materials research. Part A

Article Title: Evaluation of the tissue response to alginate encapsulated islets in an omentum pouch model

doi: 10.1002/jbm.a.35769

Figure Lengend Snippet: Immunohistochemical staining around alginate microbeads. Spleen was used as a control for markers CD68 (A), CD163 (D), CCR7 (G). CD68+ stain is observed (B, C)as well as CCR7+ (H,I). CD163+ staining was not observed (E, F). (C, F, I) higher resolution images of the inset area.

Article Snippet: The secondary antibody was added Biotinylated anti-mouse IgG (Vector, BA-2001, for CD68 and CD163) and Bio-tinylated anti-rabbit IgG (Vector, BA-1000, for CCR7) at a dilution of 1:200 for all antibodies.

Techniques: Immunohistochemical staining, Staining

Journal: Journal of biomedical materials research. Part A

Article Title: Evaluation of the tissue response to alginate encapsulated islets in an omentum pouch model

doi: 10.1002/jbm.a.35769

Figure Lengend Snippet: Masson’s Trichrome (A, B) indicates the presence of a chronic inflammatory response (red color) around both stable and failed alginate microbeads with islets and the presence of collagen for a small subset of alginate microbeads without islets. CCR7 stain indicates the presence of inflammatory macrophages (M1) in stable and failed alginate microbeads (C,D) while CD163 pro-healing macrophage phenotype (M2) was not observed in the groups with islets (E), the presence of pro-healing macrophage (CD163+) is observed in stable and failed alginate microbeads without islets (F). Arrows indicate macrophage presence.

Article Snippet: The secondary antibody was added Biotinylated anti-mouse IgG (Vector, BA-2001, for CD68 and CD163) and Bio-tinylated anti-rabbit IgG (Vector, BA-1000, for CCR7) at a dilution of 1:200 for all antibodies.

Techniques: Staining

Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: Macrophage-Derived Tumor Necrosis Factor-α Is an Early Component of the Molecular Cascade Leading to Angiogenesis in Response to Aortic Injury

doi: 10.1161/atvbaha.111.223917

Figure Lengend Snippet: Figure 6. Macrophage ablation impairs TNF-induced VEGF production and angiogenesis. A and B, Whole mount prepara- tions of rat aorta immunostained for CD163 demonstrated depletion of macrophages in clodronate-treated explants (B) compared with phosphate-buffered saline control (A). The same results were obtained with other macrophages markers (data not shown). C to F, Macrophage-depleted aortic rings failed to generate an angiogenic response (D) compared with control rings (C) and produced a reduced number of microvessels when treated with TNF (F) compared with TNF-treated rings con- taining macrophages (E). G and H, Bar graphs show differences in angiogenesis (G, days 6 to 7, n4) and VEGF production (H, n3) between macrophage-depleted rings and control rings treated with TNF or left untreated. I, The residual proangio- genic effect of TNF in clodronate treated cultures was not affected by antibody-mediated inhibition of VEGF. Scale bars100 m (A and B), 500 m (C to F). *P0.05, **P0.01, ***P0.001.

Article Snippet: Mouse monoclonal antibodies against rat CD68 (ED1) and CD163 (ED2) were purchased from AbD Serotec (Raleigh, NC).

Techniques: Saline, Control, Produced, Inhibition